Atividade funcional de fagócitos do sangue humano pela adsorção de IgA à microesferas de polietilenoglicol (PEG)

Abstract

Polyethylene glycol (PEG) polymer is an important pharmacological research modulation and operating in the prolongation of drug action. PEG microspheres are described in the literature for having adsorption properties of organic compounds, which can modify and improve biological functions. Several studies have reported the importance of hormones, plants and even immunoglobulins in functional activation of phagocytes. The antibodies are circulating proteins that have main functions neutralization and opsonization of microorganisms. IgA acts mainly in mucosal immunity and immune cell activation. Some studies suggest that IgA has opsonize particles of capacity and enhance phagocytosis and is still capable of inducing leukocytes to release oxygen derived radicals. The aim of this work was to produce and characterize PEG microspheres promote the adsorption of IgA in the PEG surface, verify action of PEG and IgA system on the rheological properties of blood and verify the functional activity of mononuclear phagocytes (MN) human blood by superoxide anion release, phagocytosis and microbicidal activity. PEG microspheres were produced and these were adsorbed IgA molecules. The placement of the IgA PEG microspheres were able to reduce blood viscosity at rates equivalent to blood control which allows IgA activity in biological systems without causing changes in viscosity. IgA Nanodoses adsorbed to PEG microspheres determine immunomodulatory effects on phagocytes of human blood when exposed to enteropathogenic Escherichia coli observed by superoxide anion release, phagocytosis and microbicidal activity. These data suggest that IgA alters functional activity of human peripheral blood phagocytic, and microbicidal increasing the phagocytosis activity of these cells and therefore this PEG microsphere with IgA system have potential neomaterial for future clinical application.