Atividade funcional de fagócitos do sangue periférico humano na presença de um sistema microemulsionado de óleo de babaçu

Abstract

The use of plants as a therapeutic resource is generalized in the popular medicine. Among the plant species used for this purpose babassu protrudes and its oil. On the other hand, microemulsions (ME) have been used as delivery vehicles for modified drugs or herbal. However the babassu oil activity, as well as its activity in microemulsion system about phagocytes from the blood have not yet been elucidated. The objective was to develop and characterize one material microemulsified babassu oil and its effect on the functional activity of phagocytes. Microemulsions were prepared from the mixture of surfactants: sorbitan monooleate (SP), polysorbate 80 (TW) and 1-butanol (BT), the babassu was used as an oil phase component and, finally, water was added. The amount of each component is related to the value of hydrophile-lipophile balance (HLB) equal or near the oil phase. Pseudoternarios diagrams were prepared and regions of diagrams of the microemulsion (ME) were defined and pre-selected. Microemulsions were characterized by rheometry and physicochemical tests. The interaction between the microemulsion and babassu oil with blood phagocytes was analyzed by releasing superoxide, phagocytosis and microbicidal activity. The (SP / TW / BT) system in the fraction of (4.2: 4.8: 1.0) resulted in HLB required by the oil phase and produced diagrams with wide range of microemulsion system. The developed formulation was classified as O / A, presents biocompatible pH, profile Newtonian, and linear viscosity. There was an increase in the release of superoxide by phagocytes MN when incubated with oil and with microemulsion of the babassu. The oil and microemulsion of the babassu increased phagocytosis. The microbicidal activity of phagocytes increased in the presence of oil after 24 hours. The microemulsion reduced the microbicidal activity of phagocytes from the blood. The results of this study suggest that babassu oil is a candidate functional material capable to active the cellular pro-oxidative mechanisms, and important alternative for future applications in therapy, especially for infectious diseases.