Efeitos de nanodoses de Bário adsorvidos a microesferas de polietilenoglicol (PEG) sobre cocultura de células mononucleares do sangue humano e linhagens de células de câncer de mama (MCF-7)

Abstract

In recent decades due to the increasing number of diseases related to heavy metals, there has been great interest in the biological effects of these compounds. Among these heavy metals, Barium (Ba) is highly toxic because it has a high potential for absorption by the organism. Based on this need to improve human health on exposure to contaminants, systems have been invested in modulating immune responses Several works have shown the associated use of PEG microspheres with chemical compounds to act in the treatment of infections and some diseases, such as cancer. On the other hand, barium has been shown to be a potent toxic agent for human cells, causing high rates of apoptosis. It is possible that adsorption of barium to PEG microspheres may interfere with the toxic actions of barium. Thus, this work aimed to evaluate the effects of barium nanodegradation adsorbed on polyethylene glycol (PEG) microspheres in human blood mononuclear cells and breast cancer cells (MCF-7). The adsorbed barium PEG microspheres were characterized and their effects on blood viscosity, cell viability and apoptosis were verified. The PEG microspheres were shown to have spherical, stable structures capable of adsorbing, regardless of the dose, BaCl2 and with a size corresponding to 7,0 μm. It was found that barium nanodoses did not alter the viscosity of normal blood. When evaluating cell viability in the presence of different doses of barium, a significant difference was observed in MCF-7 cells with a higher index for BaCl2 25pg/L and a reduction in cell viability when in coculture, especially when the cells were treated with BaCl2 1ng/L. There was an increase in apoptosis of cells incubated with BaCl2 1ng/L adsorbed to PEG microspheres, and the highest apoptosis index (85.99%) was observed in cell culture. These data suggest that BaCl2 1ng/L adsorbed to PEG microspheres may act as functional material with antitumor activity on MCF-7 cells.