Ativação e proliferação de linfócitos do colostro humano na presença de quimiocinas

Abstract

Breast milk is rich in immunocompetent cells capable of producing cytokines and chemokines which have a modulating effect on the newborn's immune system has been reported that these cells present in human milk contains cytokines and chemokines. Chemokines play a crucial role in the movement of the body mononuclear cells and their migration to the tissues, thus contributing to the adaptive immune response and pathogenesis of various diseases. This study aims to evaluate the concentration of chemokines in supernatants of human colostrum and its influence on lymphocyte proliferation. Colostrum samples were obtained from 30 healthy mothers, aged between 18 and 35 years. The concentrations of chemokines present in the supernatant of colostrum, colostrum immunophenotyping and proliferation of the cells was assessed by flow cytometry. It was observed that the supernatant of colostrum had high concentrations of MCP-1 and IP-10. An increase in the percentage of cells expressing CD4, regardless of previous stimulation after ten hours of culture. The chemokine MCP-1 increased the proliferation rate of the lymphocytes colostrum. This study suggests that MCP-1 activity on the proliferation of T lymphocytes colostrum may be an important adaptive immunity protection mechanism given to newborns during breastfeeding.