Quantificação de Porphyromonas gingivalis após o uso tópico de IgY- anti gingipaínas em cães com doença periodontal

Abstract

This study aimed to evaluate the effectiveness of IgY-anti gingipaínas, through the detection and quantification of Porphyromonas gingivalis (Pg) in the oral cavity of dogs with stage I periodontal disease (PD), and there effects on visual card indexes (IP), calculus (IC) and gingivitis (IG). 30 dogs were used, namely GT (treatment group) for those used IgY- anti gingipaínas (200 mg/day), GP (placebo group) and GC (control group). Subgingival curettage was performed in 3 days where T1 (preceded the mechanical cleaning), T2 (immediately after mechanical cleaning) and T3 (28 days after mechanical cleaning). Curettage products have undergone qualitative polymerase chain reaction (PCR) for Pg and Fusobacterium spp (Fs) and quantitative (qPCR) to Pg, the visual analysis at T3 to the groups. In T3, GP and GT showed reduction in the number of PG compared to GC, the detection of Fs was lower in GT when compared to GP and GC, no difference was noted between groups over IP, IC and IG. The topical use of IgY-anti gingipaínas not change the number of Pg, but promoted reduction in the number of positive animals to Fs, what is a evolution mediator of PG on the DP. Different approaches are proposed for prevention of early dogs, DP once periodontitis installed is not reversible, even after successful therapies. These results encourages studies with IgY- anti gingipaíns.