Correlação da amamentação com níveis de citocinas e imunofenotipagem de células T regulatórias no sangue de mulheres com câncer de mama

Abstract

Cancer is a public health problem, and the impact of breast cancer on the population correspond to 80% of the more than 20 million new cases of cancer estimated by 2025. Epidemiological evidence suggests that breastfeeding reduces the risk of breast cancer by virtue of The differentiation of the mammary tissue, reduction in the number of ovulatory cycles and the immunological components present in the secretion between these cells and cytokines. Thus, the objective of this study was to evaluate the serum levels of cytokines (IL8, IL-1β, IL-6, IL-10 and TNF-α) and the phenotyping of regulatory T cells present in the blood of women with breast cancer And correlate with the time of breastfeeding. Peripheral blood samples were collected from women with no evidence of breast cancer (Control Group n = 8) and women with confirmed diagnosis for breast cancer (Study Group n = 12). Participants answered a semi-structured questionnaire to evaluate aspects related to breastfeeding. Cytokines and phenotyping were evaluated by flow cytometry. It was observed that the time of breastfeeding was higher in the control group when compared to the experimental group. There was an increase in the concentration of IL-6, IL-8 and TNF-α in the blood of women with breast cancer. It is emphasized that there was a correlation between the time of breastfeeding and the levels of IL-8 in the study group. Regardless of the breast tumor, the percentage of regulatory T cells was similar between the groups. The data suggest that breast cancer conditions a profile of inflammatory cytokines in the blood, and that increased breastfeeding time exert protective effect against breast cancer.