Suplementação do meio de criopreservação de sêmen de bovino pantaneiro com vitaminas C e E

Abstract

The objective of this work was to evaluate the effect of the addition of vitamin C and / or Trolox (water soluble analogue of vitamin E) on parameters related to the fertility of cryopreserved semen of Pantaneiro bulls (Bos taurus) breeds in central Brazil. Six Pantaneiros bulls were used, from which semen was collected and divided into T1 Control: only the base diluent (Triladyl® + egg yolk); T2: base diluent with addition of 0.6 mg / ml Vitamin C. T3: base diluent + 0.3 mg / ml Vitamin E / Trolox and T4: base diluent + 0.6 mg / ml Vitamin C + 0 , 3 mg / ml Vitamin E / Trolox. After thawing, the analyzes of motility, vigor, sperm viability, acrosomal membrane integrity, mitochondrial cytochemical activity, induced and spontaneous lipid peroxidation, in vitro embryo production and analyzed for movement quality (CASA) were performed. The experiment was carried out in a completely randomized design with data analyzed through Anava and Tukey and Wilcoxon tests, with a significance level of 5%. The mitochondrial activity was reduced (P <0.0001) with the addition of antioxidants, especially the interaction between control, vitamin C, E and association for DAB 1 (41.33 ± 4.13 vs 6.52 ± 2.20 vs 5.41 ± 1.52 vs 32.50 ± 6.02) and increased (P <0.0001) the number of DAB 3 (9.00 ± 1.84 vs 33.79 ± 3.56 vs 50.00 ± 5.98 vs 17.50 ± 2.38), respectively. In the in vitro production of embryos no difference was observed, independent of the treatments used (P> 0.05). It is concluded that the level of antioxidants used may not have been sufficient to express an improvement in seminal quality and decrease the levels of reactive oxygen species, thus leading to a greater decrease in mitochondrial activity.