Efeitos da associação do Hibiscus Sabdariffa e do treinamento físico sobre a capacidade funcional, resistência insulínica e esteatose hepática em ratos obesos

Abstract

Hibiscus Sabdariffa (HS) has been widely used for weight loss and to combat comorbidities associated with obesity. This study evaluated the effect of HS extract and physical training on functional capacity, insulin resistance and hepatic steatosis in obese rats. Wistar rats were distributed in: control (C), treated with Hibiscus Sabdariffa (HS), high intensity interval training (TI), TI + HS (TIHS), Obese (O), obese + continuous aerobic training (OTA), obese + HS (OHS), obese + TA + HS (OTAHS). Obese rats received a high-calorie diet for 16 weeks. The extract of Hibiscus Sabdariffa was administered for 60 days at a dosage of 150 mg / kg of body weight. Maximum progressive effort test (MGET) was performed. The variables analyzed were: maximum speed (Vmax), time and distance covered. Lactate was measured immediately after MGET. Interval training was 80% of Vmax for 8 minutes and 20% of Vmax for 2 min during 60 minutes for 60 days. Aerobic training was 50% of the Vmax during 60 minutes for 60 days. A glucose tolerance test was performed by administering a 2.5g / kg glucose solution via gavage and samples were collected at times 0, 15, 30, 60.90 and 120 minutes, liver analysis of the lipid and glycogen profile. Insulin resistance was assessed by the curve profile and glycemic area. Data analyzed by ANOVA and Bonferroni test, p <0.05. The hypercaloric diet promoted an increase in weight gain, excess body fat, hyperglycemia, hepatic steatosis and insulin resistance. Aerobic training and high intensity intervals reduced final body weight, weight gain and adiposity index, as well as increased Vmax, Time and distance covered in MGET and improved functional capacity. TA reduced food intake, insulin resistance and hepatic steatosis in obese rats. Hs supplementation reduced the adiposity index, food intake, body fat, reduction in hepatic steatosis and insulin resistance in obese rats. In eutrophic rats, supplementation with Hs decreased the adiposity index. The association of Hs and TA reduced the final body weight, weight gain, food intake and increased functional capacity. In conclusion, Hs supplementation promoted a reduction in the adiposity index in eutrophic and obese rats and increased the functional capacity of trained obese rats. There was no association of Hs and TF on hepatic steatosis and insulin resistance, indicating that the effects of TF and Hs act independently in reducing liver steatosis and insulin resistance.