Correlação de parâmetros hematológicos e de citocinas com a viscosidade sanguínea levando-se em consideração o sexo dos cães positivados para leishmaniose canina visceral

Abstract

In this dissertation, dogs naturally infected with Leishmania infantum demonstrated a decrease in blood viscosity and serum concentration of Tumor of Tumor Necrosis (TNF-alpha) as the cytokine responsible for this rheological alteration, as well as one of the causes of the reduction of erythrocytes. Rheometry of fresh blood samples was performed using a compact modular rheometer - MCR 102, and graphs were obtained using the Rheoplus software. Blood count data were obtained using an automated Icounter 3D hematology analyzer. Diagnostic confirmation was obtained by Elisa immunoenzymatic serological assay to determine the groups not infected and infected by Leishmania infantum. Serum cytokines were measured by flow cytometry (FACScalibur BD®) using BD® Biosciences Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine kits. The results showed a correlation between blood viscosity (p < 0.05) and TNF-alpha in the group of sick animals that presented it, and that changes in this rheological parameter are correlated with the sex of the evaluated dogs. Thus, these findings are unprecedented in the consulted literature. Which may also suggest an alternative for monitoring dogs with Canine Visceral Leishmaniasis and the search for more effective therapies.