Correlação entre parâmetros bioquímicos e soropositividade para diferentes genótipos de Ehrlichia canis em Cuiabá-MT

Abstract

Proteins with tandem repeats proteins from Ehrlichia canis are immunoreactive (TRPs) and were used in the serological diagnosis of canine monocytic ehrlichiosis (CME). In the same samples, biochemical parameters were analyzed to assess renal, hepatic and muscle function. The purpose of the present study was to evaluate the use of synthetic peptides referring to the immunoreactive positions of TRP19, USTRP36U, BrTRP36 and CRTRP36 proteins in enzyme-Liked immunosorbent assay (ELISA) to evaluate possible associations between biochemical alterations in dogs naturally infected with E. canis. Two hundred sera were submitted to ELISA with antigens of the TRP19, USTRP36, BrTRP36 and CRTRP36 proteins of E. canis. Sera were also evaluated using eight biochemical parameters and the results were evaluated by principal component analysis and canonical correlation. ELISA revealed that 47 (23.5%) serum samples reacted to the BrTRP36 peptide, 36 (18%) to the TRP19 peptide, 8 (4%) to the USTRP36 peptide and 8 (4%) to the CRTRP36C peptide. The moust outstanding diagnostic method in the canonical correlation analysis was BrTRP36 followed by TRP19, which were correlated with hyperglobulinemia and hypoalbuminemia, suggesting that the Brazilian genotype may be associated with immunopathological disorders observed in the course of the disease in dogs. The results of the canonical analysis, combined withphysiological characteristics of each dependent variable analyzed, demonstrate that the joint reactivity of the TRP19 and TRP36 peptides is associated with the chronic phase of infection by E. canis in the studied region.