Estudo epidemiológico da parvovirose canina e caracterização genômica de um isolado do tipo 2C no município de Cuiabá-MT

Abstract

Canine parvovirus is a icosahedral non-enveloped DNA virus responsible for enteritis and myocarditis in dogs that was first identified in the 1970s and rapidly spread throughout the world. To date, three antigenic variants were described (CPV-2a/b/c). This study was conducted with the objective to isolate the canine parvovirus from a sample of feces of an infected dog from the Midwest region of Brazil following by the sequencing of the whole genome and it´s applicability in an hemagglutination inhibition assay to detect antibodies against CPV in a collection of serum samples of a population of dogs. Nonenveloped icosahedral Parvovirus-like were isolated in CRFK cells. Genomic sequence followed by phylogenetic analysis of DNA positioned our isolate in a clade comprised by strains of CPV-2c. The deduced amino acid sequence of the VP2 capsid protein showed the common changes found in CVP-2c variant including the glutamic acid amino acid in the residue 426. The hemagglutination inhibition assay (cut-off: ≥80) using the CPV-2c isolate as antigen showed a high occurrence (97.2%; 310/320) when tested in a sera bank. Tendency for positivity was observed among dogs until 12 months’ age (p=0.08). The present study reports for the first time an isolate of CPV-2c with the whole genomic characterization in Brazil.