Desenvolvimento e caracterização de microesfera de polietilenoglicol (PEG) adsorvida com melatonina extraída do colostro humano e seus efeitos sobre a atividade funcional de fagócitos do colostro

Abstract

Polyethylene glycol (PEG) are synthetic polymers known for their biocompatibility, water solubility and the ability to alter the properties of other polymers or molecules. Melatonin is a neurohormone present in various secretions, such as blood and colostrum, which works in multiple functions that are linked to cellular processes. PEG microspheres in their various forms are a highly reliable alternative because they tend to reduce invasive treatments, such as in the case of hydrogel and transdermal systems. PEG microspheres are capable of modulating the functional activity of colostrum phagocytes, but there is no data on the activity of melatonin present in colostrum. Thus, the objective of this study was to extract melatonin from human colostrum, develop and characterize PEG microspheres with melatonin extracted from colostrum adsorbed, as well as evaluate the effects of this system on the oxidative stress of colostrum phagocytes. Thirty colostrum samples were collected, of which 10 were used for melatonin extraction and 20 colostrum samples for phagocyte extraction. The polyethylene glycol microspheres produced were analyzed by fluorescence microscopy and flow cytometry. Oxidative stress was assessed by the release of superoxide anion and superoxide enzyme. A control was used using commercial melatonin. Fluorescence microscopy and flow cytometry analyses revealed that the PEG microsphere was able to adsorb the PEG microsphere. These data highlight the importance of the natural origin of melatonin on oxidative stress and suggest that colostrum melatonin may be a more efficient option to control the oxidative index.