Efeito de diferentes concentrações de pentoxifilina adicionada ao sêmen de bovinos da raça Pantaneiro pós descongelamento

Abstract

The present study aimed to evaluate the effects of adding different concentrations of pentoxifylline to the semen of Pantaneiro bulls post-thaw. Semen samples were collected from four Pantaneiro bulls, cryopreserved, and stored in a cryogenic tank. After thawing, the samples were analyzed for sperm motility (MOT), vigor (VIG), mitochondrial activity (DAB), and oxidative stress (TBARS). The samples were divided into four aliquots and incubated with one of four pentoxifylline (PTX) treatments: Control (no PTX), T1 (1.75 mM PTX), T2 (3.75 mM PTX), and T3 (5.25 mM PTX). Evaluations of the aforementioned parameters were performed after 20, 40, 60, 120, and 180 minutes of incubation. No significant differences (P > 0.05) in MOT and VIG were observed among the treatments. However, significant variations were identified across incubation times, with higher motility and vigor recorded during the first 40 minutes. Mitochondrial activity also declined progressively with increasing incubation time. Conversely, oxidative stress levels (TBARS) did not differ significantly among treatments or incubation times. These findings suggest that while pentoxifylline did not significantly impact long-term sperm parameters, it had a beneficial effect on semen motility and vigor during the initial minutes following thawing.