Desenvolvimento e caracterização de um sistema de liberação modificada para incorporação de melatonina com atividade moduladora de fagócitos e células tumorais da mama

Abstract

The new materials (neomaterials) microemulsified associated with polyethylene glycol are being promising alternatives for modified release of active pharmacological substances, improving therapeutic effects and reducing side effects. Microemulsified systems, having adequate properties that favor the incorporation of substances and intracellular absorption, are being intensively used in the pharmaceutical industry. The melatonin hormone associated with the release system may be a viable alternative as an immunomodulator of the oxidative stress of phagocytes and neoplastic cells. The aim of this study was to develop and characterize a microemulsified neomaterial associated with polyethylene glycol, as an alternative for the release of melatonin as a modulating agent for the oxidative stress of mononuclear cells (MN) and breast adenocarcinoma cell lines (MCF-7). The microemulsified systems were developed from the surfactants polysorbate 80 (TWEEN 80) and polyethylene glycol 400 (PEG 400), aqueous phase and isopropyl myristate as oily phase (MLP), later associated with melatonin (MLM) and carried out their physical-chemical characterizations and rheological. Hemorrheological tests and evaluation of cellular oxidative stress were performed using melatonin (MLT) and liquid microemulsified system associated with PEG 400 and melatonin (MLM) as stimuli. Points of the MLP neomaterials showed desirable physical-chemical and rheological characteristics for biological assays. MLM showed changes in the flow curve and blood viscosity and MCF-7 cells and immunomodulatory effects on oxidative cell stress, while MLT did not show changes in blood behavior, but it did have a modulating effect on the oxidative stress of MCF-7 cells. It is concluded that the modified release MLM neomaterial may be a possible immunomodulator as an alternative for breast cancer therapy.