Avaliação do processo de cicatrização de lesões de queimadura de segundo grau tratadas com membrana de borracha natural e copaíba

Abstract

The present study aimed to evaluate whether treatment with natural rubber-copaiba (BNC) membranes can influence the healing process of 2nd degree burns in Wistar rats. The wounds were evaluated for their contraction and histological appearance. In addition, ERK 1/2 activation, TLR4, TNF-R1 and CB2 receptor expression and TNFα concentration were quantified. Male Wistar rats (N=22) were divided into 5 groups: control group without treatment (N=7), sulfadiazine group, treated with silver sulfadiazine (N=5) NRC group, trated with natural rubber and Copaíba(N=5) and normal skin group, without burn. The treatments started immediately after the burn induction and were requested for 10 days. The macroscopic analysis of the lesion revealed a white and uniform wound, and on the 10th day after the burn, all groups presented crust formation. Treatment with BNC significantly increased the wound contraction rate (33,8±1,882%; p<0,0001) and showed improvements in the histological aspect of the wound, in addition, it was able to significantly increase the activity of ERK 1/2 (p= 0,0009) while reducing the expression of TLR4 (p=0,0147), TNF-R1 (p=0,0021) and the concentration of TNFα (p=0,0002), indicating a possible reduction in pro-inflammatory activity followed by an increase in cell proliferation and differentiation. In conclusion, the treatment of 2nd degree burns with BNC was responsible for reducing TNFα concentration and increasing ERK 1/2 activity, promoting greater contraction and reduction of inflammatory activity during the proliferative phase of healing.