Cateterização uretral após indução farmacológica para coleta de sêmen em cães

Abstract

Erection and ejaculation are influenced for α-adrenergic agents, and ejaculatory reflex is primarily an α-adrenergically mediated event. This paper aims to describe the semen pharmacological collection by urethral catheterization technique in domestic dogs using dexmedetomidine (Dexdomitor, Zoetis, Finland) and ketamine (Ketalex, Vencofarma, Brazil) association. For dose determination, semen collection was performed using dexmedetomidine increasing doses (5μg/kg, n=2; 10μg/kg, n=2 and 15μg/kg, n=2) associated to 3mg/kg ketamine in six animals. Once the dose was established, additional semen collection using 15μg/kg dexmedetomidine and 3mg/kg ketamine was carried out in nine dogs. After the procedure, sperm volume (0.087 ± 0.027 mL), total motility (60.5 ± 8.08%), progressive motility (1.9± 0.28), sperm concentration (2458 ± 1303.44 x 10⁶ sperm / mL), sperm viability (84.2 ± 13.97%), acrosomal integrity (75.2 ± 18.83 %) and normal sperm morphology (57.35 ± 6.49 %) were assessed. According the results, this study demonstrates that urethral catheterization for semen collection using dexmedetomidine-ketamine association can be performed in dogs and it may be possible to obtain semen for application in biotechnologies, despite urine contamination.