Diversidade de vírus RNA em glândulas salivares de culicíneos do Parque Nacional de Chapada dos Guimarães, Mato Grosso, Brasil

Abstract

Metagenomic studies in mosquitoes contributed with a great diversity of new viral variants and insect specific viruses (ISV), with substantially divergent genomes of the previously known viruses, promoting revisions on current viral classifications and suggesting the inclusion of new taxa and families. The present study proposed the viral diversity identification in salivary glands of wild mosquitoes captured in a Cerrado area of Chapada dos Guimarães National Park, Mato Grosso, Brazil. In total, 66 Culicinae female mosquitoes belonging to at least 16 species were captured and allocated to nine pools after salivary glands dissection. These pools were subjected to viral RNA extraction, second-strand of cDNA synthesis, PCR randomic amplification and high-throughput sequencing (Illumina HiSeq 2500). Results revealed the presence of seven ISV associated with salivary glands of different mosquito species, six of which are new species belonging to Rhabdoviridae (Lobeira virus), found in a pool of Stegomyia albopicta, Chuviridae (Cumbaru and Croada virus) in Mansonia wilsoni and Psorophora spp. respectively, Totiviridae (Murici virus) in Ma. wilsoni and Partitiviridae (Araticum and Angico virus) in Ma. wilsoni and Culex sp.. Also, the recently discovered Kaiowa virus, found in St. albopicta and Ochlerotatus, previously reported in Culex sp. in Pantanal of Mato Grosso do Sul. These findings contribute to knowledge of viral diversity in wild culicines, promoting more precise reviews of involved phylogenetic groups, as recently originated Dielmovirus (Rhabdoviridae) and Artivirus (Totiviridae) genera, the new family Chuviridae and wide variety of recently described viruses not classified yet in genera within the family Partitiviridae.