Avaliação do papel do Glucagon e do AMPc na produção de glicose, glicólise e glicogenólise no fígado de ratos alimentados com farinha de soja

Abstract

Poor nutritional environment in early life is related to increased susceptibility to glucose intolerance or type 2 diabetes mellitus during adult life and soybean utilization is indicated as being useful to prevention of these disorders. Due to its recommendation, our studies are focused to the action of soybean as recovery method for animal model of protein restriction during early life. However, our results are conflicting with the vision of potential positive effect of the diet based soybean, since it resulted in reduced level of hepatic glycogen and increased serum ALT. Thus, considering these results we investigated if the diet and/or the nutritional state could affect the action of glucagon and cAMP on glucose production, glycolysis and glycogenolisis by the liver perfusion technique.Male offspring from mothers fed with 17% or 6% protein (casein) during pregnancy and lactation were maintained with 17% casein (CC and LC groups) or soybean (CS and LS groups) diet and with 6% casein (LL group) diet until 90 days of life.Liver of recovery groups (LC and LS rats) has responded more effectively to cAMP actionin compare to LL group, suggesting higher sensitivity to glicolitic agent.Through cAMP perfusion, soybean diet groups showed increased glucose production, higher glycolysis inhibitionwithout significative increase in glycogenolisis. Thus, we suggest that the glucose produced by liver of soybean groups is, at least in part, recycled to be used in the glycogen synthesis as a manner of ensure some glycogen content in relation to control diet groups.