Desenvolvimento e caracterização de um neomaterial microemulsionado contendo extrato de quina do cerrado (Strychnos pseudoquina) para aplicação em células tumorais

Abstract

Microemulsions can be defined in a broad sense, as a homogenous system of water and oil dispersions, where a lipophilic drug and an oil is dispersed in an aqueous medium or, stabilized by an emulsifier, with or without the an appropriate co-emulsifier. The microemulsions study can provide therapeutic alternatives pharmacologically more efficient and more reduced side effects and possess the ability to enhance the solubility of hydrophilic and lipophilic substances, to increase the stability and the absorption of stray drugs, allowing increased bioavailability, It can also provide a prolonged effect. The pseudoquina Strychnos is a native tree of the Brazilian Cerrado, known as the Cerrado Quina, several pharmacological actions are attributed to this species, being used for various therapeutic purposes. Thus, the objective of this work was to study the physical and chemical stability of microemulsion

formulations prepared with binary and ternary mixtures of surfactant / co- surfactant, the stability of formulations after the incorporation of the Cerrado

Quina and assess the apoptosis of tumor cells breast modified in the presence of developed system. Distilled water was used, triglyceride / capric caprylic acid (oil phase), and oletado sorbitan monolaurate, Polysorbate 80 and 1-butanol (surfactants), the system being referred to as SP / TW / BT. After 72 hours at 25 ° C the samples were visually evaluated, and separated into liquid emulsion regions (EL) emulsion gel (EG), liquid ME (MEL) gel ME (MEG), and phase separation (SF). The extract of the Cerrado Quina was obtained by maceration of 416 g of dry powder plant in 3000 ml of ethanol P.A., followed by distillation. They used the systems in the region MEL and performed physical, chemical and rheological tests, being chosen the points 3M, 5M, 6M and 7M for incorporation of the Cerrado Quina extract, and again performed stability tests. The microemulsion 7M was considered the most stable with Newtonian characteristics and biological assays were performed with apoptotic tumor cells MFC-7 breast using flow cytometry. The modified system showed antitumor effect in the presence of MCF-7 cells and normal, and a possible therapeutic alternative for the treatment of this disease.