Prevalência e diversidade genética de Trypanosoma theileri em bovinos leiteiros de Ji-Paraná, Rondônia

Abstract

Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that is infectious for ruminants from different countries. Through this study we investigated the prevalence of the agent in cattle from 64 farms of family farms, divided into 06 rural sectors in accordance with Technical Assistance and Rural Extension Association of the State of Rondônia. We also analyzed the genetic diversity of T. theileri isolates of cattle in the municipalities of Ji-Paraná. DNA from 320 blood samples and 13 isolates of T. theileri from cows underwent diagnostic PCR based on amplification of the Cathepsin L-like protein (CATL) gene and glycosomal glyceraldehyde genes 3- phosphate dehydrogenase (gGAPDH) and a small subunit rRNA (V7V8 SSU rRNA) region only for isolates. The prevalence in cattle was 8,13% (95% IC: 6,18%- 10,59%), demonstrating the T. theileri circulation in the municipality. Then with phylogenetic analyzes with the amplicons of CATL, gGAPDH and V7V8 SSU rRNA genes we verified the first time the presence of genotypes TthIB in catlle from western Amazon and TthIF in cattle from Brazil. Indeed, we identified a possible new genotype TthIL de T. theileri.