Listeria monocytogenes em carne bovina produzida em Mato Grosso, Brasil : ocorrência, caracterização genotípica e perfil de resistência a antibióticos e sanitizantes

Abstract

Listeria monocytogenes is a foodborne pathogen that causes listeriosis, a serious zoonosis, that affects at-risk populations, such as pregnant women, the elderly and immunocompromised women, and presents high mortality rates (20% to 30%). This bacterium is ubiquitous and has the ability to form biofilms, grow at refrigeration temperatures, develop mechanisms of resistance to sanitizers used in the food industry and antimicrobial compounds, tolerate acidic conditions and high concentrations of salt, making it difficult to control them in the processing environment meat. This work presents a literature review that characterizes the contamination of bovine meat by L. monocytogenes and an experimental study carried out to determine the occurrence of this microorganism in fresh bovine meat produced in the state of Mato Grosso - Brazil, to identify the serotypes and characterize the profile of antimicrobial resistance to antibiotics and also the main sanitizers used in slaughterhouses. A total of 50 samples, produced by 13 different slaughterhouses, were submitted to bacteriological analysis, six strains of L. monocytogenes were isolated from six different samples, the isolates were submitted to antibiogram tests, the determination of the minimum inhibitory concentration of sanitizers (CIM), serotyping and molecular characterization by PFGE method. The occurrence was found to be 12%, serotypes 4b and 1 / 2b were identified and the resistance of the strains to sulfonamides and sodium hypochlorite was verified.