Vigilância de doenças virais em aves de subsistência criadas próximas a sítios de aves migratórias no estado de Mato Grosso

Abstract

In Mato Grosso, in the counties of Cáceres and Araguaiana there are areas of interface between migratory birds and backyard poultry. Circulation of avian influenza virus (AIV), Newcastle disease virus (NDV), avian metapneumovirus (aMPV), and avian infectious bronchitis virus (IBV) are unknown in these sites due to the scarcity of studies. The objective of this work was to conduct an serum epidemiological survey on backyard poultry for these agents that cause economic and social losses in commercial poultry worldwide. Samples (serum, cloacal swabs and trachea swabs) of 1068 birds, among chickens, quails, turkeys, ducks and geese, were analyzed for AIV and NDV by ELISA, Hemagglutination Inhibition and RT-PCR in real-time. For aMPV and IBV were analized 623 chickens in the ELISA test. The frequency sample positive of ELISA for AIV, NDV, aMPV and IBV in Araguaiana were 0.7%, 23%, 85% and 69%, respectively; whereas in Cáceres the frequency was 0.8%, 43.5%, 88.5% and 70%, respectively. Only in Caceres there was a duck (0.6%) positive for HI for NDV. There were no PCR reagent samples for AIV and NDV. The spatial analysis demonstrated the agglomeration of properties with NDV seropositive birds within the urban zone of Cáceres, suggesting the participation of this area in viral dissemination. This study evidenced infection by viral pathogens in backyard poultry in the site of migratory birds in the state of Mato Grosso, demonstrating the importance of these birds as sources of infection for other birds.