Ação da mangaba (Hancornia speciosa) adsorvida em polietilenoglicol (PEG) sobre células do câncer de mama (MCF-7)

Abstract

The action of biomolecules in the human organism can be limited by two factors: diffusion speed and period of degradation. The adsorption of these biomolecules in polyethylene glycol (PEG) based microspheres allows to reduce degradation and ensure controlled release over time. The objective of this work was to evaluate the antioxidant activity of mangaba extract nanodoses adsorbed to the PEG microspheres on the coculture of human blood mononuclear cells (MN) and MCF-7 cell lines. PEG microspheres were characterized by flow cytometry. The cell viability was evaluated by fluorescence microscopy and the antioxidant activity was determined by the release of superoxide anion (O2 - ), production of the enzyme copper zinc Superoxide Dismutase (Cu-Zn-SOD) and free radical capture: DPPH, ABTS and FRAP. The content of total phenolic compounds (CFT) and total carotenoids, as well as rheological parameters of blood and possible changes in blood viscosity in the presence of ethanolic extract (EE) of the fruit adsorbed to PEG were determined. EE was obtained by maceration in ethyl alcohol followed by distillation 20% PEG microspheres were prepared. The solutions were then adjusted (PEG microsphere and EE, v.v-1

), and incubated at 37°C for 30 minutes. Release of

O2 - was determined using the chromogen Ferricitochrome C (2 mg.mL-1

) and SOD was measured by the initial rate of reduction of Nitro Blue Tetrazolium (NBT). PEG microspheres with stable, spherical structures capable of adsorbing mangaba EE and having a size corresponding to 6,6 μm were produced. In the presence of EE there was no reduction in cell viability and changes in blood viscosity. The values of DPPH (14,84 ± 0,00 μmol TE.g-1 ),

ABTS (16,70 ± 0,83 μmol TE.g-1

), FRAP (52,53 ± 1,70 μmol Fe2 SO4.g-1

), total carotenoids

(0,24 ± 0,01mg β-carotene.100g-1

) and CFT (208, 81 ± 20.13 mg GAE.100g-1

) were expressive with data from the literature. The EE of the fruit adsorbed to PEG microspheres showed increase in O2 - levels (2,49 nmol to 3,41 nmol) in MCF7 and MN cell cultures. SOD activity in MCF-7 cells treated with mangaba EE declined relative to the control treatment (94,77 NBT.SOD-1 to

77,68 NBT.SOD-1

) and increased co-culture of MCF-7 cells and cells MN treated with PEG- adsorbed mangaba EE (64,07 NBT.SOD-1

to 85,03 NBT.SOD1

). These results suggest that PEG microsphere-adsorbed mangaba EE increased the functional activity of human blood cells and MCF-7 cell culture and MN cells, and could therefore act as functional material with antitumor activity on MCF- 7.