Alteração de perfil do microbioma (16srdna) no rúmen de ovinos alimentados com Brachiaria decumbens

Abstract

Brachiaria spp. is the most used forage in Brazilian pastures. In spite of its importance in cattle ranching, grasses of this genus can lead to intoxication in several species, causing photosensitization in sheep, goats, cattle and horses, as well as slimming frames in sheep without the presence of dermatitis, which leads to economic losses significant. Factors related to both herds and individuals are important in the occurrence of photosensitization. The pathogenesis of intoxication is associated with the saponins present in Brachiaria, which are capable of generating hepatocellular lesions that later lead to photosensitization. The saponins present the ability to affect the microbial profile of the digestive systems of ruminants, this alteration may be determinant for the development of individual resistance. We performed 16S rDNA amplification profile studies to identify bacterial populations and their variation in animals before and after feeding with Brachiaria to investigate the pathogenesis of this intoxication. Ruminal fluid samples from five sheep were collected in three periods, one before and the other two after feeding with Brachiaria. the obtained DNA was submitted to new generation sequencing. Our results indicate that the consumption of Brachiaria was able to promote changes in the ruminal microbial ecosystem.